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Conformational and quantitative characterization of lysozyme extracted from galyfilcon and senofilcon silicone hydrogel contact lenses.

Glasier MA, Keech A, Sheardown H, Subbaraman LN, Jones L

Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.

Purpose: To compare two solvents for retrieval of lysozyme deposited on a silicone hydrogel (SH) contact lens material galyfilcon A (GA; Acuvue Advance). Methods: Two buffers used were 50:50 acetonitrile/0.02% trifluoroacetic acid (buffer 1) and 50:50 acetonitrile/50 mM NaOH (buffer 2). Results: Extraction efficiency from GA lenses was 74% (buffer 1) and 83% (buffer 2). Buffer 2 decreased lysozyme activity > buffer 1. Ex vivo GA lenses showed total protein deposition of 2-16 mu g/lens with total lysozyme deposition of 0.3-3.9 mug/lens. Conclusions: We have developed a low acid strength extraction buffer that can be used to efficiently extract active lysozyme protein from novel siloxane-based contact lens materials.

Published 24 January 2008 in Curr Eye Res, 33(1): 1-11.
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